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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/03/2009 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JOSÉ, A. A. F. B. V.; GAMA, M. A. S.; LANNA, D. P. D. |
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Afiliação: |
A. A. F. B. V. José, ESALQ/USP; Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; Dante Pazzanese Duarte Lanna, ESALQ/USP. |
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Título: |
Effects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 7, n. 2, p. 284-294, 2008. |
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DOI: |
https://doi.org/10.4238/vol7-2gmr417 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue. MenosThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12... Mostrar Tudo |
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Palavras-Chave: |
Adipose tissue explants; Bioactive fatty acid. |
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Thesaurus Nal: |
conjugated linoleic acid; lipogenesis; swine. |
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Categoria do assunto: |
-- |
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URL: |
https://www.funpecrp.com.br/gmr/year2008/vol7-2//pdf/gmr417.pdf
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Marc: |
LEADER 02768naa a2200217 a 4500
001 1596283
005 2024-02-08
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol7-2gmr417$2DOI
100 1 $aJOSÉ, A. A. F. B. V.
245 $aEffects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs.$h[electronic resource]
260 $c2008
520 $aThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.
650 $aconjugated linoleic acid
650 $alipogenesis
650 $aswine
653 $aAdipose tissue explants
653 $aBioactive fatty acid
700 1 $aGAMA, M. A. S.
700 1 $aLANNA, D. P. D.
773 $tGenetics and Molecular Research$gv. 7, n. 2, p. 284-294, 2008.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/12/2011 |
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Data da última atualização: |
14/02/2018 |
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Autoria: |
GONZATTO, M. P.; KOVALESKI, A. P.; BRUGNARA, E. C.; WEILER, R. L.; SARTORI, I. A.; LIMA, J. G. de; BENDER, R. J.; SCHWARZ, S. F. |
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Afiliação: |
Mateus Pereira Gonzatto, Programa de Pós-graduação em Fitotecnia/Universidade Federal do Rio Grande do Sul/Faculdade de Agronomia/Departamento de Horticultura e Silvicultura; Alisson Pacheco Kovaleski, Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Centro de Pesquisa para Agricultura Familiar; Eduardo Cesar Brugnara, Programa de Pós-graduação em Fitotecnia/Universidade Federal do Rio Grande do Sul/Faculdade de Agronomia/Departamento de Horticultura e Silvicultura; Roberto Luis Weiler, Programa de Pós-graduação em Fitotecnia/Universidade Federal do Rio Grande do Sul/Faculdade de Agronomia/Departamento de Horticultura e Silvicultura; Ivar Antônio Sartori, Instituto Federal de Educação/Ciência e Tecnologia Catarinense; Jurandir Gonçalves de Lima, Fazenda Panoramas Citrus; Renar João Bender, Programa de Pós-graduação em Fitotecnia/Universidade Federal do Rio Grande do Sul/Faculdade de Agronomia/Departamento de Horticultura e Silvicultura; Sergio Francisco Schwarz, Programa de Pós-graduação em Fitotecnia/Universidade Federal do Rio Grande do Sul/Faculdade de Agronomia/Departamento de Horticultura e Silvicultura. |
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Título: |
Performance of 'Oneco' mandarin on six rootstocks in South Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 4, p. 406-411, abril 2011 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Comportamento da tangerineira 'Oneco' sobre seis porta?enxertos no Sul do Brasil. |
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Conteúdo: |
The objective of this work was to assess the effect of six rootstocks on yield, fruit quality, and growth of 'Oneco' mandarin during the first seven harvesting seasons, in Butiá, Rio Grande do Sul State, Brazil. The rootstocks evaluated were: 'Swingle' citrumelo (Citrus paradisi × Poncirus trifoliata), 'Caipira' orange (C. sinensis), 'Troyer' citrange (C. sinensis × P. trifoliata), 'Rangpur' lime (C. limonia), 'Volkamer' lemon (C. volkameriana), and 'Flying Dragon' trifoliata orange (P. trifoliata var. monstrosa). Plants budded onto 'Flying Dragon' had the lowest vegetative development, which indicates the dwarfing characteristics of this rootstock, and had the highest mean production efficiency, despite low yield. Plants grafted on 'Volkamer' lemon and 'Rangpur' lime had the highest alternate bearing. Under the experimental conditions evaluated, the most adequate rootstocks for mandarin 'Oneco' are 'Swingle' citrumelo and 'Troyer' citrange, regarding fruit yield and quality. |
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Palavras-Chave: |
Alternância de produção; Qualidade de fruto. |
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Thesagro: |
Citrus Reticulata; Produtividade. |
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Thesaurus NAL: |
Alternate bearing; Fruit quality; Yields. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172565/1/Performance-of-Oneco-mandarin-on-six.pdf
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Marc: |
LEADER 01938naa a2200301 a 4500
001 1910166
005 2018-02-14
008 2011 bl uuuu u00u1 u #d
100 1 $aGONZATTO, M. P.
245 $aPerformance of 'Oneco' mandarin on six rootstocks in South Brazil.
260 $c2011
500 $aTítulo em português: Comportamento da tangerineira 'Oneco' sobre seis porta?enxertos no Sul do Brasil.
520 $aThe objective of this work was to assess the effect of six rootstocks on yield, fruit quality, and growth of 'Oneco' mandarin during the first seven harvesting seasons, in Butiá, Rio Grande do Sul State, Brazil. The rootstocks evaluated were: 'Swingle' citrumelo (Citrus paradisi × Poncirus trifoliata), 'Caipira' orange (C. sinensis), 'Troyer' citrange (C. sinensis × P. trifoliata), 'Rangpur' lime (C. limonia), 'Volkamer' lemon (C. volkameriana), and 'Flying Dragon' trifoliata orange (P. trifoliata var. monstrosa). Plants budded onto 'Flying Dragon' had the lowest vegetative development, which indicates the dwarfing characteristics of this rootstock, and had the highest mean production efficiency, despite low yield. Plants grafted on 'Volkamer' lemon and 'Rangpur' lime had the highest alternate bearing. Under the experimental conditions evaluated, the most adequate rootstocks for mandarin 'Oneco' are 'Swingle' citrumelo and 'Troyer' citrange, regarding fruit yield and quality.
650 $aAlternate bearing
650 $aFruit quality
650 $aYields
650 $aCitrus Reticulata
650 $aProdutividade
653 $aAlternância de produção
653 $aQualidade de fruto
700 1 $aKOVALESKI, A. P.
700 1 $aBRUGNARA, E. C.
700 1 $aWEILER, R. L.
700 1 $aSARTORI, I. A.
700 1 $aLIMA, J. G. de
700 1 $aBENDER, R. J.
700 1 $aSCHWARZ, S. F.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 46, n. 4, p. 406-411, abril 2011
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Embrapa Unidades Centrais (AI-SEDE) |
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